Integrative teaching
Ciclo Di Seminari
Exercises
Examination procedure
Oral exam
Prerequisites
IV and V year students
Syllabus
Overview of the cell as a complex physical system -The cell structures -Fluorescence microscopy: scooping the biological world The cell’s life -Equilibrium thermodynamics of the cell -Protein folding, protein binding, allostery -Excited-state modulation of fluorescence to image molecular interactions i. Forster Resonance Energy Transfer ii. Fluorescence depolarization -Non-Equilibrium thermodynamics of the cell: the local formulation and steady states -Biological membranes: life in two dimensions -Molecular crowding, confinement -Biological dynamics and diffusion iii. Diffusion on membranes iv. Nucleocytoplasmic diffusion -Relaxation and fluctuation methods to probe biomolecular dynamics v. Fluorescence Recovery After Photobleaching vi. Fluorescence Correlation Spectroscopy -Exchanges with outside world - vii. Ion exchanges and biological electricity viii. Free-energy transduction -Far from equilibrium systems and dissipative structures -Dynamics of molecular motors -Metabolic regulation and non-linear enzymes -Single Particle tracking Multicellular organisms -The origin of biological altruism -Epigenetics and tumorigenesis -The tissue regulation of glucose homeostasis -Fingerprints of cell and tissues ix. Fluorescence lifetime imaging of endogenous co-factors x. Fluorescence biosensors
Bibliographical references
Phillips et al. Physical Biology of the Cell