Lecture
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24 Nov 2023 (2h 00m)
Federico Cremisi - Course (teaching activity) - Face to face
Lesson 1. The molecular composition of chromatin: histone and non histone proteins. The nucleosome and the logical of chromatin folding. Nucleosome sliding and histone chaperons. The position variegation effect of drosophila is at the bases of the discovery of epigenetics and of the screening of genes responsible for histone modifications. Histone modification readers and writers. Insulators. The heterochromatin spreading. Pionier trascription factors and the first positioning of chromatin modifications.
29 Nov 2023 (2h 00m)
Federico Cremisi - Course (teaching activity) - Face to face
Lesson 2. The nucleosomic composition of the centromeric etherochromatin. The bases of epigenetic memory in early development. The evidence of the constance of the genome in vertebrates. Nuclear transfer experiment in Xeopus. The evidence of epigenetic memory in nuclear transplants. The role of Histon 3 isoforms in developmental epigenetic memory of master genes.
06 Dec 2023 (2h 00m)
Federico Cremisi - Course (teaching activity) - Both face to face and online
Lesson 3. Control of gene expression: from the textbook (Alberts 7th edition), pgs 513-515 (NGS); pgs 397- 409 414-423 (Chapter 7, Control of gene expression in eukariotes)
15 Dec 2023 (2h 00m)
Federico Cremisi - Course (teaching activity) - Face to face
Lesson 4. MOLECULAR GENETIC MECHANISMS THAT CREATE AND MAINTAIN SPECIALIZED CELL TYPES.. GRADIENTS AND THRESHOLDS CREATE STRIPES OF EXPRESSION IN DROSOPHILA. COOPERATIVE AND COMBINATORIAL CONTROL OF TRANSCRIPTION. GENE REGULATORY NETWORKS. FEEDBACK AND FEED FORWARD REGULATION CIRCUITS. THE LESSON OF ERIC DAVIDSON.
15 Jan 2024 (2h 00m)
Federico Cremisi - Course (teaching activity) - Face to face
Lesson 5. MECHANISMS THAT REINFORCE CELL MEMORY IN PLANTS AND ANIMALS. POST-TRANSCRIPTIONAL CONTROLS: SPLICING, POLYADENILATION. (Alberts 7h, Pgs. 435-461).
19 Jan 2024 (2h 00m)
Federico Cremisi - Course (teaching activity) - Face to face
Lesson 6. RNA STABILITY. REGULATION OF GENE EXPRESSION BY NON CODING RNAs. (Alberts 7th pgs 460- 471). A microRNA involved in the evolution of cerebral cortex. Paolino et al., PNAS 2020, selected figures . Martins et al., Stem Cells Reports 2021, selected figures.
26 Jan 2024 (2h 00m)
Federico Cremisi - Course (teaching activity) - Face to face
Lesson 7. The role of Chromatin conformation in gene expression, The historical approaches for the study of interphase chromatin conformation: lumpbrash and polythene chromosomes. The rainbow staining of interphasic chromosomes by FISH. Methods of chromatin capture conformation. The description of interphasic chromatin nucleoplasm structural and functional compartmentalisation. Globular fractal organisation of the interphasic chromatin.
29 Jan 2024 (2h 00m)
Federico Cremisi - Course (teaching activity) - Face to face
Lesson 8. Chromatin reorganization at the nuclear lamina orchestrates developmental gene regulation. A basal chromosome architecture present in embryonic stem cells is cumulatively altered at hundreds of sites during lineage commitment and subsequent terminal differentiation. Chromatin remodeling involves both individual transcription units and multigene regions and affects many genes that determine cellular identity. Lamina-genome interactions are widely involved in the control of gene expression programs during lineage commitment and terminal differentiation.
31 Jan 2024 (2h 00m)
Federico Cremisi - Course (teaching activity) - Face to face
Lesson 9. The location of chromosomes from and to the nucleus: a way to control tissue-specific gene expression. NE transmembrane proteins (NETs), are mostly tissue-specific proteins that stabilized by its own structural protein network the nuclear lamina. Expression of distinct NETs increase the incidence of the lacO array at the nuclear periphery with high statistical significance. Tissue-restricted NETs play a pivotal role in dissecting the functional relevance of tissue-specific patterns of radial chromosome positioning in development.
09 Feb 2024 (2h 00m)
Federico Cremisi - Course (teaching activity) - Face to face
Lesson 10. Analyzing Cells, Molecules, and Systems (Alberts 7th, pgs 475-542, selected topics). ISOLATING CELLS AND GROWING THEM IN CULTURE. STUDYING GENE EXPRESSION AND FUNCTION.
10 Apr 2024 (2h 00m)
Alessandro Cellerino - Course (teaching activity) - Online
Metodi di analisi degli acidi nucleici. Cap 8 Alberts con approfondimenti su tecniche di next generation sequencing e RNAseq
17 Apr 2024 (2h 00m)
Alessandro Cellerino - Course (teaching activity) - Face to face
Metodi di purificazione e analisi degli acidi nucleici. Capitolo 8 dell'Alberts con approfondimenti su spettrometria di massa e proteomica
24 Apr 2024 (2h 00m)
Alessandro Cellerino - Course (teaching activity) - Both face to face and online
Capitolo 8 ALberts: genetica classica e tecniche di "reverse genetics", animali transgenici
30 Apr 2024 (2h 00m)
Alessandro Cellerino - Course (teaching activity) - Face to face
Capitolo 9 Alberts. Tecniche di imaging
08 May 2024 (2h 00m)
Alessandro Cellerino - Course (teaching activity) - Face to face
Capitolo 8 modelli matematici dei meccanismi di regolazione genica
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