Examination procedure
Oral exam
Prerequisites
PhD students
Syllabus
- A primer on regulation of gene expression and statistical considerations
- RNA-seq: how it works
- Sequence quality control and mapping algorithms
- Detection of differential gene expression
- Clustering and other methods to reduce dimensionality
- Analysis of differential expression using DESeq2
- Gene Ontology and Gene Set Enrichment
- Analysis of GO terms and pathways using WebGestalt
- Network analysis
- Applications of network analysis to the brain
- Cell-specific transcriptome analysis in the brain
- Single-cell RNA-seq, methods and analysis
- Single-cell RNA-seq, applications to the nervous system
- Methods for spatial transcriptomics
- Analysis of microRNAs and RNA immunoprecipitation
- , Proteome analysis by mass-spectroscopy
- Analysis of synaptic proteome
- Analysis of nascent proteins and RNAs
- Epigenetic analysis: DNA methylation and ChIP-seq
- Examples of epigenetic studies in the nervous system
Bibliographical references
Cellerino & Sanguanini "Transcriptome analysis" Ed. Scuola Normale