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Biologia e Fisiologia Molecolare della Cellula

Registro delle lezioni

Anno accademico 2024/2025
Docente Alessandro Cellerino, Federico Cremisi, Luca Dolfi

Lecture

  • 21 Nov 2024 (2h 00m)

    FEDERICO Cremisi - Corso (attività didattica) - In presenza

    Lesson 1. The molecular composition of chromatin: histone and non histone proteins. The nucleosome and the logical of chromatin folding. Nucleosome sliding and histone chaperons. The position variegation effect of drosophila is at the bases of the discovery of epigenetics and of the screening of genes responsible for histone modifications. Histone modification readers and writers. Insulators. The heterochromatin spreading. Pionier trascription factors and the first positioning of chromatin ,,,

  • 28 Nov 2024 (2h 00m)

    FEDERICO Cremisi - Corso (attività didattica) - In presenza

    Biologia e Fisiologia Molecolare della Cellula - Lesson 2. The nucleosomic composition of the centromeric etherochromatin. The bases of epigenetic memory in early development. The evidence of the constance of the genome in vertebrates. Nuclear transfer experiment in Xeopus. The evidence of epigenetic memory in nuclear transplants. The role of Histon 3 isoforms in developmental epigenetic memory of master genes.

  • 05 Dic 2024 (2h 00m)

    FEDERICO Cremisi - Corso (attività didattica) - In presenza

    Lesson 3. Control of gene expression: from the textbook (Alberts 7th edition), pgs 513-515 (NGS); pgs 397- 409 414-423 (Chapter 7, Control of gene expression in eukariotes)

  • 12 Dic 2024 (2h 00m)

    FEDERICO Cremisi - Corso (attività didattica) - In presenza

    Lesson 4. MOLECULAR GENETIC MECHANISMS THAT CREATE AND MAINTAIN SPECIALIZED CELL TYPES.. GRADIENTS AND THRESHOLDS CREATE STRIPES OF EXPRESSION IN DROSOPHILA. COOPERATIVE AND COMBINATORIAL CONTROL OF TRANSCRIPTION. GENE REGULATORY NETWORKS. FEEDBACK AND FEED FORWARD REGULATION CIRCUITS. THE LESSON OF ERIC DAVIDSON.

  • 13 Dic 2024 (2h 00m)

    FEDERICO Cremisi - Corso (attività didattica) - In presenza

    Lesson 5. MECHANISMS THAT REINFORCE CELL MEMORY IN PLANTS AND ANIMALS. POST-TRANSCRIPTIONAL CONTROLS: SPLICING, POLYADENILATION. (Alberts 7h, Pgs. 435-461).

  • 09 Gen 2025 (2h 00m)

    FEDERICO Cremisi - Corso (attività didattica) - In presenza

    Lesson 6. RNA STABILITY. REGULATION OF GENE EXPRESSION BY NON CODING RNAs. (Alberts 7th pgs 460- 471). A microRNA involved in the evolution of cerebral cortex. Paolino et al., PNAS 2020, selected figures . Martins et al., Stem Cells Reports 2021, selected figures.

  • 15 Gen 2025 (2h 00m)

    FEDERICO Cremisi - Corso (attività didattica) - In presenza

    Lesson 7. The role of Chromatin conformation in gene expression, The historical approaches for the study of interphase chromatin conformation: lumpbrash and polythene chromosomes. The rainbow staining of interphasic chromosomes by FISH. Methods of chromatin capture conformation. The description of interphasic chromatin nucleoplasm structural and functional compartmentalisation. Globular fractal organisation of the interphasic chromatin.

  • 16 Gen 2025 (2h 00m)

    FEDERICO Cremisi - Corso (attività didattica) - In presenza

    Lesson 8. Chromatin reorganization at the nuclear lamina orchestrates developmental gene regulation. A basal chromosome architecture present in embryonic stem cells is cumulatively altered at hundreds of sites during lineage commitment and subsequent terminal differentiation. Chromatin remodeling involves both individual transcription units and multigene regions and affects many genes that determine cellular identity. Lamina- genome interactions are widely involved in the control of gene express..

  • 22 Gen 2025 (2h 00m)

    FEDERICO Cremisi - Corso (attività didattica) - In presenza

    Lesson 9. The location of chromosomes from and to the nucleus: a way to control tissue-specific gene expression. NE transmembrane proteins (NETs), are mostly tissue-specific proteins that stabilized by its own structural protein network the nuclear lamina. Expression of distinct NETs increase the incidence of the lacO array at the nuclear periphery with high statistical significance. Tissue- restricted NETs play a pivotal role in dissecting the functional relevance of tissue-specific patterns of ...

  • 23 Gen 2025 (2h 00m)

    FEDERICO Cremisi - Corso (attività didattica) - In presenza

    Lesson 10. Analyzing Cells, Molecules, and Systems (Alberts 7th, pgs 475-542, selected topics). ISOLATING CELLS AND GROWING THEM IN CULTURE. STUDYING GENE EXPRESSION AND FUNCTION.

  • 06 Feb 2025 (2h 00m)

    LUCA Dolfi - Corso (attività didattica) - In presenza

    tecniche di analisi delle proteine

  • 12 Feb 2025 (2h 00m)

    LUCA Dolfi - Corso (attività didattica) - In presenza

    tecniche di analisi del DNA

  • 13 Feb 2025 (2h 00m)

    LUCA Dolfi - Corso (attività didattica) - In presenza

    tecniche di analisi molecolare del fenotipo e del genotipo e tecniche di transgenesi

  • 12 Mar 2025 (2h 00m)

    LUCA Dolfi - Corso (attività didattica) - In presenza

    tecniche di microscopia di base

  • 19 Mar 2025 (2h 00m)

    LUCA Dolfi - Corso (attività didattica) - In presenza

    tecniche di microscopia avanzata

  • 07 Apr 2025 (2h 00m)

    ALESSANDRO Cellerino - Corso (attività didattica) - In presenza

    Modelli matematici della regolazione genica

  • 15 Apr 2025 (2h 00m)

    ALESSANDRO Cellerino - Corso (attività didattica) - In presenza

    Introduzione all'Evoluzione

  • 22 Apr 2025 (2h 00m)

    ALESSANDRO Cellerino - Corso (attività didattica) - Mista

    Ricostruzione alberi filogenetici

  • 16 Maggio 2025 (2h 00m)

    ALESSANDRO Cellerino - Corso (attività didattica) - In presenza

    Utlizzo di MegaX per ricorstruzione alberi filogenetici

  • 23 Maggio 2025 (2h 00m)

    ALESSANDRO Cellerino - Corso (attività didattica) - In presenza

    Natura e misura della selezione

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