Stem Cells 1

04 Mar 2024 (2h 00m)

Federico Cremisi - Corso (attività didattica) - In presenza

Lesson 2. The nature of the radial glial progenitor cell. Lateral inhibition in flyes and vertebrates. Neurogenic and proneural genes. The role of Notch signaling in vertebrate neurogenesis.

11 Mar 2024 (2h 00m)

Federico Cremisi - Corso (attività didattica) - In presenza

Lesson 3. The adult neuronal stem cell niche. B, C and A type cells. The role of VCam1, Notch, BMP and Shh in the stem cell niche maintenance. Extrinsic signals from blood and cerebrospinal fluid regulating the niche.

20 Mar 2024 (2h 00m)

Federico Cremisi - Corso (attività didattica) - In presenza

Lesson 4. Studies on the mechanisms of action of the SHH signaling in maintaining the SVZ neural stem cell niche. Transgenic approaches to identify NSC cell progeny and analyze its composition in longitudinal studies. SHH signaling promotes the maintainance and increases the number of quiescent NSCs, while depleting the activated NSCs. SHH signaling affects NSCs by shorthening the G1 cell cycle phase.

08 Apr 2024 (2h 00m)

Federico Cremisi - Corso (attività didattica) - In presenza

Lesson 5. SHH and the adult hippocampal niche. Long-lived NSCs dramatically expand in the first postnatal week before entering the quiescent state over several days. Sufu deletion impairs the ability of long-lived NSCs to expand in the first postnatal week, which results in the premature entry of NSCs into the quiescent state. This defect is a result of decreased Shh signaling activity as a result of Sufu deletion in NSCs. Highly activated mossy cells increase NSC proliferation. Seizure-induced neurogenesis is attenuated by selective deletion of Shh in mossy cells. Shh from mossy cells is needed to preserve the NSC pool after seizure-induced neurogenelsis. Deletion of Shh results in premature depletion of the NSC pool after seizures.

15 Apr 2024 (2h 00m)

Federico Cremisi - Corso (attività didattica) - In presenza

Lesson 6. Other adult stem cells: intestinal, hematopoietic, mesenchimal cells (from Developmental Biology, Gilbert, 9th edition, pgs 158-167).

22 Apr 2024 (2h 00m)

Federico Cremisi - Corso (attività didattica) - In presenza

Lesson 7. The Human Model System to Study Development and Disease::starting from mouse embryonic stem cells and proceeding through human cell reprogramming to pluripotency..Gilbert, Developmental Biology, 166--177. Selected papers: Qi-Long Ying et al. Nature 2008.; Warmflash et al., Nature 2014; Ghimire et al., Scientific Report 2017.

23 Apr 2024 (2h 00m)

Federico Cremisi - Corso (attività didattica) - In presenza

Lesson 8. The molecular nature of pluripotent stem cells. Pervasive transcription, relaxed chromatin and enhanced RNA interference of chromatin remodellers are peculiar traits of embryonic stem cells Selected papers: Takahashi et al., Cell 2006; Efroni et al., Cell Stem Cell 2008; Huangfu et al., Nature Biotechnology 2008; Pandolfini et al., Genome Biology 2014.