Didattica integrativa
Ciclo Di Seminari
Esercitazioni
Modalità d'esame
Prova orale
Prerequisiti
General and basic knowledge of molecular and cell biology and of physiology.
Students of other disciplines are most welcome and any requirement of previpus knowledge can be discussed individually.
Programma del corso
Programma
Didattica integrativa (24 hours/12 Lectures)
1. Ionic channels-introduction and general properties.
2. Classification of voltage gated channels and their functional role.
3. Membrane potential: Nernst and Goldman equation
4. Passive properties of the membrane/Active properties: the Action Potential and the Hodgkin and Huxley model.
5. Synaptic transmission general properties of chemical and electrical synapses.
6. Quantal analysis
7. Classical neurotransmitters systems-neuropeptides.
8. Integrative properties of the synapse.
9. Neurotransmitter release- quantal theory - probability of release/ Molecular mechanisms of NT release.
10. Ionotropic receptors.
11. Metabotropic receptors and Neurotransmitter transporters.
12. New insights on the role of microglia in the regulation of synaptic transmission.
Main Course
1. Introduction to synaptic plasticity (Hebb’s rule, LTP, LTD, BCM theory).
2. Short term plasticity (paired pulse facilitation, paired pulse depression)
3. Transition from short- to long-term plasticity: the role of de novo transcription and translation
4. Activity-dependent transcription. Rapid calcium-dependent transcription of immediate early genes. c-fos, Arc, BDNF, Npas4. CREB and late response genes.
5. Axonal and dendritic targeting of mRNAs.
6. Local protein synthesis in the presynaptic and the post-synaptic compartments
7. Biochemical pathways linking synaptic activity to translation initiation
8. Fragile X mental retardation protein (FMRP) and LTD
9. Synaptic tagging and capture. Cross-tagging.
10. Inverse synaptic tagging. Functions of Arc
11. Homeostatic plasticity. Spike time dependent plasticity
12. Generalities on memory. Memory traces and engram cell assemblies. Consolidatio.n and reconsolidation
13. Genetic tagging of active neurons.
14. Neuronal assemblies or synaptic assemblies?
15. Erasing specific memories: necessity of engram cell assemblies
16. Instating specific Sufficiency of
17. False memories. Fully synthetic memories. Switching emotional valence to memories
18. Direct testing of the synaptic plasticity theory of memory.
19. Synaptic engrams and experimental methods to study them
20. Introduction to neuronal development (1)
21. Introduction to neuronal development (2)
21. Critical periods for synaptic plasticity
23. Adult neurogenesis and memory
24. Regeneration of CNS neurons
Riferimenti bibliografici
TextBook Martin, Brown, Diamond, Cattaneo, De-Miguel "From Neuron to Brain" 6th Edition 2021 Oxfors University Press
Integrated with papers from the literature